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Dnase I Solution : Tribo™ DNase I Solution

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DNase I (RNase-free)は、DNAを非特異的に切断するエンドヌクレアーゼであり、5´-リン酸化末端と3´-ヒドロキシル化末端を有するジヌクレオチド、トリヌクレオチド、オリゴヌクレオチドを遊離する。DNase Iは、1本鎖および2本鎖DNA、クロマチンやRNA:DNAハイブリッドに作用する。 Product Description Deoxyribonuclease (DNase) I Solution (1 mg/mL) is useful for reducing or preventing aggregation of concentrated and/or cryopreserved cell suspensions following thawing. The activity range is ≥ 2000 units per mg of protein.

DNase I Solution, 1mg/ml , 5x1ml

(Positive control slide: in DNase I solution (100µl of 200µg/ml), 10 min, RT. 1x PBS rinse, 2 times in a separate container then combine with other slides.) 6. Deoxyribonuclease (DNase) I Solution (1 mg/mL) is useful to reduce or prevent the clumping of concentrated and/or cryopreserved cell suspensions following thawing.

DNase I (RNase free) - GeneON BioScience

DNase I is a recombinant endonuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, to release di-, tri- and oligonucleotide products Solution 1 unit μL RNase (on average producing tetranucleotides) with 5’-phosphorylated and 3’-hydroxylated ends. It acts on single-stranded DNA, double-stranded DNA, RNA-DNA hybrids and chromatin. DNase I

DNase I 溶液（1 单位/μL），无 RNase. Thermo Scientific DNase I removes unwanted DNA from cell lysates to improve protein extraction efficiency. Features of RNase-free DNase I: Degrades and removes unwanted DNA from samples Cleaves both s. Available in 1000 单位 Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5′-phosphat. DNase I Solution (Catalog #07900) is provided with an activity range of ≥ 2000 units per mg of protein. Thus, the starting concentration of DNase I may be assumed to be 2000 units/mL for the purposes of these calculations.

DNase I是一种需二价阳离子的脱氧核糖核酸内切酶，可用于降解单链或双链 DNA。 其原理为 DNase Ⅰ水解磷酸二酯键产生带有5′-磷酸基团和3′-OH的单核苷酸或寡核苷酸。

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Solution de DNase I (1 unité/μl), exempte de RNase. La DNase I Thermo Scientific élimine l’ADN indésirable des lysats cellulaires afin d’améliorer l’efficacité de l’extraction des protéines.

DNase I Solution (1 unit/μL), RNase-free. Thermo Scientific DNase I removes unwanted DNA from cell lysates to improve protein extraction efficiency. Features of RNase-free DNase I: Degrades and removes unwanted DNA from samples Cleaves both s. Available in 1000 Units Deoxyribonuclease I (DNase I) グリコシル化したポリペプチド鎖とジスルフィド結合2つを含むエンドヌクレアーゼ、デオキシリボヌクレアーゼⅠです。 separate container then combine with Directions for Use DNase I can be reconstituted in water or buffers (pH 4.5 – 9.0), such as neutral balanced salt solutions (e.g. phosphate-buffered saline; Hanks‘ Balanced Salt Solution). The choice of buffer and concentration is application-specific; however, higher stock solution concentrations (such as 1 mg/mL) are recommended.

Tribo™ DNase I Solution

Recipe DNase I solution Dilute a preparation of RNase-free DNase I to a concentration of 10 units/μl using DNase dilution buffer. Store the solution at -20°C. One unit is defined as the amount of DNase that will cause a change in A 260 of 0.001/minute/ml of a reaction using a If a solution is protease-free, DNase I will not loose significant activity at pH 5 – 7 and 62°C for 5 hours. The enzyme may be heat-inactivated (10 minutes at 99°C).

Product Description Deoxyribonuclease (DNase) I Solution (1 mg/mL) is useful for reducing or preventing aggregation of concentrated and/or cryopreserved cell suspensions following thawing. The activity range is ≥ 2000 units per mg of protein. DNase I (脱氧核糖核酸酶I)，即Deoxyribonuclease I，是一种可消化单链或双链DNA的脱氧核糖核酸内切酶，它识别并切割磷酸二酯键，产生5’端为磷酸基团，3’端为羟基的单脱氧核苷酸或单链或双链的寡脱氧核苷酸。 is also well Thermo Scientific DNase I removes unwanted DNA from cell lysates to improve protein extraction efficiency. Features of Thermo Scientific DNase I: Degrades and removes unwanted DNA from samples Cleaves both single-s

Conditions for complete DNA digestion Add 10X DNase I Buffer to 1X concentration in the solution to be DNase-treated, and add approximately 1–2 of DNase I per 1 μg DNA present.

Deoxyribonuclease (DNase) I Solution (1 mg/mL) is useful to reduce or prevent the clumping of concentrated and/or cryopreserved cell suspensions following thawing.

The DNase I Solution is provided at a concentration of 1mg/mL (2000U/mL). 10X Reaction Buffer and Stop Solution are included in the kit. Applications Elimination of the DNA template following in vitro RNA synthesis with T7 Phage RNA Polymerase. Characterization of DNA: protein interactions by one of “DNase I footprinting”. Deoxyribonuclease (DNase) Dissolve DNase I at 1 I Solution (1 mg/mL) is useful to reduce or prevent the clumping of concentrated and/or cryopreserved cell suspensions following thawing. 「DNase I (RNase free) 」 (Code No.314-08071)について、新製品「DNase I (RNase free) 」発売開始に伴い、2025年6月11日受注分をもって販売終了いたします。 製品説明 製品内容 使用例 Q & A 資料 Data Sheet 関連情報 問い合わせ先 製品説明 本品は、ウシ膵臓由来のDeoxyribonuclease Iを酵母で発現させたものです。本品

PharmaOnco™ DNase I Solution

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Recipe DNase I solution Dilute a preparation of RNase-free DNase I to a concentration of 10 units/μl using DNase dilution buffer. Store the solution at -20°C. One unit is defined as the amount of DNase that will cause a change in A 260 of 0.001/minute/ml of a reaction using a DNase I，即Deoxyribonuclease I，中文名称为脱氧核糖核酸酶I，是一种可以消化单链或双链DNA产生单脱氧核苷酸或单链或双链的寡脱氧核苷酸的核酸内切酶。DNase I水解单链或双链DNA后的产物，5’端为磷酸基团，3’端为羟基。

DNase I Solution (RNase & Protease-free) 脱氧核糖核酸酶I溶液（无RNase&蛋白酶） 当前位置： 首页> 产品中心> 分子生物学研究 > RNA提取 > DNase I Solution (RNase & Protease-free) 脱氧核糖核酸酶I溶液（无RNase&蛋白酶） RNA提取 While DNase I activity steadily DN 25 which may decreases with increasing salt concentrations, DNase I-XT remains active in solutions containing up to 300 mM salt. Figure 2: DNase I-XT removes more DNA from IVT reactions and RNA preparations

Thermo Scientific DNase I removes unwanted DNA from cell lysates to improve protein extraction efficiency. Features of RNase-free DNase I: Degrades and removes unwanted DNA from samples Cleaves both single-stranded Buffer RDD, included in the set, is optimized for on-column DNase digestion of 15 minutes at 20–30°C. The buffer is also well-suited for efficient DNase Applications Elimination digestion in solution. The RNase-Free DNase Set provides efficient on-column digestion of DNA during RNA purification from cells and tissues using RNeasy Kits and the QIAamp RNA Blood Mini Kit. 1 unit refers to the amount of DNase I required to act on 1 mg/mL of DNA (pH 5.0, 25°C) to produce an increase in absorbance of 0.001 per minute at a wavelength of 260 nm.

Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5′-phosphate and 3′-OH groups. The enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ ions. In the presence of Mg2+, DNase I If a solution is protease-free, DNase I will not loose significant activity at pH 5 – 7 and 62°C for 5 hours. The enzyme may be heat-inactivated (10 minutes at 99°C). RNase-free DNase I: Dissolve DNase I at 1 mg/ml in 0.1 M iodoacetic acid plus 0.15 M sodium acetate at a final pH of 5.3. The solution is then heated 40 minutes at 55°C and cooled.

Not all DNase I products are tested for solution appearance, but those which have specific assays are tested in 0.15 M NaCl at 5 mg/mL; clear solutions are seen for all but 溶液 1 DN-25, which may give a hazy solution. Solution stability depends on several factors: stabilizing calcium ion, concentration, temperature and protease contamination.

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RNase-Free DNase Set はRNeasy Kit およびQIAamp RNA Blood Mini Kit を用いた細胞および組織からのRNA ml of a reaction 精製の際に、カラム上でのDNase 除去を効率的に行ないます。 DNase は続いて行なう洗浄ステップで、効率的に除去されます。