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General

Nonsurgical Techniques In Laboratory Animals

Di: Amelia

Any opinions, findings, conclusions, or recommendations expressed in this pub-lication are those of the authors and do not necessarily reflect the views of the organizations or agencies that provided support for the project. The content of this publication does not necessarily reflect the views or policies of the National Institutes of Health, nor does mention of trade names, No.13/1, 3rd Seward Road, Valmiki Nagar, Thiruvanmiyur, Chennai-600 041. Good Laboratory Practices (GLP) for animal facilities is intended to assure quality maintenance and safety of animals used in laboratory studies while conducting biomedical and behavioral research and testing of products.

Guidelines on administration of substances and blood collection in

Despite general acceptance of the need to refine experiments such that animal pain and distress is minimized and a growing emphasis on laboratory animal welfare, less than 25% of laboratory However studies suggest rodents undergoing surgical procedures receive postoperative analgesics. Encouraging greater use of postoperative analgesics and implementing improvements in anesthesia and

Guide for the care and use of Laboratory Animals (TH) - Chulabhorn ...

Which of the following is an example of refinement? The use of computer modeling software to substitute for animal experiments. The shared use of tissues by more than one investigator to minimize animal use. The use of improved assays that provide better data precision, so that fewer animals are needed per group. The use of newer surgical techniques that result in less tissue Chapter 1 The Contribution of Laboratory Animals to Medical Progress—Past, Present, and Future

General Guidelines for Non-survival Surgical Procedures According to USDA Animal Care Policy #3, “Nonsurvival surgery does not require aseptic techniques or dedicated facilities.” However, there may be instances when aseptic technique is warranted. Factors such as the degree of tissue trauma, surgical site (e.g., gastrointestinal surgery), harvesting of tissues Various regulatory agencies and guidelines have restricted the use of animals and the techniques used for blood collection in laboratory animals. This article deals with the approved blood collection techniques for laboratory animals like rodents, lagomorphs and nonrodents.

Surgeons must wear a clean lab coat, gown, or scrub top and a mask. A surgical bonnet is also recommended. Sanitize hands before gloving. Note on gloves: Sterile gloves are required if you will manually manipulate animal tissues, sterile implants, the tips of your sterile instruments, and/or disinfected areas with your hands. If you will only touch the handles of your instruments Belgian Council for Laboratory Animal Science (BCLAS): this body unites all who work agencies and guidelines have restricted in laboratory animal research and also strives to promote respectful handling of laboratory animals. Personnel training is a key aspect of surgical preparation. Laboratory surgeons should be equipped with written/practical material to assist in their mastery of the proposed technique. Research personnel should review anatomic diagrams and equipment (e.g., implant) specifications and complete a sufficient quantity of practice procedures on cadaver animals

Background Federal regulations and guidelines mandate that animals undergoing potentially painful procedures be provided with adequate anesthesia and analgesia. In addition, for these procedures, a veterinarian must be consulted regarding the appropriate anesthetic and analgesic agent and dose for the species being used and the procedure being performed. The standard

Handbook of Laboratory Animal Science

Coordination of ordering and receiving with animal care personnel is important to ensure that animals are received properly and that appropriate facilities are available for housing. Several documents provide details on transportation, including the AWRs and the International Air Transport Association Live Animal Regulations (IATA 1995).

The United States Department of Agriculture’s (USDA) Animal Welfare Act (AWA) regulations, the Public Health Service’s (PHS) Guide for the Care and Use of Laboratory Animals (Guide), and Canadian Council on Animal Care’s (CCAC) Guide to the Care and Use of Ex-perimental Animals require aseptic technique be used for survival surgical procedures performed on animals.

In principle, blood sampling techniques should be selected that expose the animal to the least possible stress. Observance of these points has of th American a positive effect both on the animal (less stress) and on the quality of the blood (less stress-induced influence on blood parameters).

Nonsurgical Methods of Contraception in Dogs and Cats: Where Are We Now ...

COMMENTS & RECOMMENDATIONS Whenever it is appropriate, aseptic technique should be given preference over clean technique Characteristics of the surgical model determine the appropriateness of clean vs aseptic technique e.g. immunocompromised animals, protracted surgery, and any surgery which accesses (infection) susceptible tissue such as intra-abdominal This chapter presents an overview of the basic clinical strategies, both pharmacologic and nonpharmacologic, for managing pain in laboratory animals. Topics include preventive analgesia, consequences of unrelieved pain, and ethical considerations relating to pain as a subject of study. Available information on pain management of nonmammalian species is also presented. Recently, however, the scientific community, citing concerns about animal welfare as well as the validity and applicability of outcomes, has called the use of animals in research into question. In this review, we seek to summarize the current

Includes survival surgery, pre surgical planning, training and qualifications, aseptic techniques, surgical monitoring, post surgical care, and assessment of outcome. U.S. Government Principles for the Utilization and Care of Vertebrate Animals Used in Testing, Research, Teaching, and Training VIII. Investigators and other personnel shall be appropriately Anesthesia and analgesia (A&A) are the main components of many procedures on laboratory animals. However, studies suggest that a considerable number of researches are based on improper use or reporting of A&A methods [1, 2]. In a study on 400 articles that included surgical models in mouse and other species, the authors concluded that the animal A&A are

Abstract The use of a nonsurgical embryo transfer technique in rodents eliminates the potential pain, distress, and health complications that may result from a surgical procedure and as such, represents a refinement in rodent assisted reproductive techniques. A nonsurgical technique has not been previously developed for use with rat embryos.

Administration of substances to laboratory animals requires careful consideration and planning to optimize delivery of the agent to the animal while minimizing potential adverse experiences from the procedure. For all species, many different routes are available for administration of 1. PREFACE Arising from a proposal by the Laboratory Animals Science Association, the Councils of the Royal Society and the Universities Federation for Animal Welfare (UFAW) set up a Steering Group that invited groups of specialists to prepare guidelines, setting out basic principles for the conduct of animal experiments and for the care of stock, to be used by all persons I. Introduction The Public Health Service (PHS) policy based on Public Law requires that institutions receiving grant or contract funds from the PHS adhere to the “Guide for the Care and Use of Laboratory Animals” in studies involving animal subjects. The “Guide” establishes the use of aseptic techniques to prevent clinical infections, during surgery procedures. In addition, the

European Academy of Laboratory Animal Surgery (EALAS) is the „go-to“ association for information about non-clinical surgery and surgical training in Europe. COMMENTS / RECOMMENDATIONS Whenever it is appropriate, aseptic technique should be given preference over clean technique Characteristics of the surgical model determine the appropriateness of clean vs aseptic technique e.g. immunocompromised animals, protracted surgery, and any surgery which accesses (infection) susceptible tissue such as intra-abdominal

Justification for Applying Aseptic Technique in Rodent Surgery The importance of maintaining asepsis (NRC Guide for the Care and Use of Laboratory Animals): Although mice and rats have been touted as being resistant to post-surgical infections, the literature contains numerous articles that document how subclinical infections such as Pseudomonas aeruginosa, Corynebacterium Animal research is considered a key element in advance of biomedical science. Although its use is controversial and raises ethical challenges, the contribution of animal models in medicine is essential for understanding the physiopathology and novel treatment alternatives for several animal and human diseases. Current pandemics’ pathology, such as the 2019 All technical support staff, physicians and post-doctoral trainees working with animals will be expected to review this MANUAL, with special attention given to those aspects of animal care policy and procedure relevant to their work environment. It is the direct responsibility of the principal investigator to ensure that personnel working in animal research have reviewed this

The innovative technique involving the use of ovarian arteries instead of uterine arteries (as in the standard protocol) was used in laboratory animals to develop the surgical protocol for transplantation of reproductive tissues. Introduction These guidelines describe ‘aseptic surgical technique’ and so outline Personnel training is a the measures the AEC expects investigators to take in order to minimise the chance of infections in animals undergoing surgical or other invasive techniques. The use of aseptic technique for animal survival surgery is prescribed in the Australian code for the care and use of animals for scientific

Scope These guidelines apply to all surgical procedures performed on rodents at the NIH in which the animals are expected to recover from anesthesia. Prior to performing any survival surgery techniques on rodents, an approved Animal Study Proposal (ASP) must be in place with descriptions of the surgical procedures to be performed and the anesthesia/analgesia1,2 that o laboratory animals: routes of administration and factors to consider. Journal of th American Association for Laboratory Animal Science modeling software to substitute for r, B.K. and Foust, K.D., 2014. Intravenous injections in onatal mice. JoVE (Journal of Visualized Experiments e, and humane method for sub ndibular bleeding of mice using a lancet. Lab animal, Surgery 5 Pre-surgical planning & pre-operative care 5 Aseptic technique 6 Preparation of surgical instruments and the animal 8 Preparation of the surgeon 10 Draping and instrument handling 10 Suture selection 10 Post-operative care 11 Facilities for aseptic surgery 12 Maintenance of the surgical facility 14

This is the ninth volume in a series dealing with induced lesions in laboratory animals. The information on pathology and toxicology Factors such as the degree documented in the series is an aid to scientific institutions, industry and government agencies charged with the

Blood is collected from laboratory animals for various scientific purposes, for example, to study the effects of a test drug on various undergoing surgical or other constituents, such as hormones, substrates, or blood cells. In the field of pharmacokinetics and drug metabolism, blood samples are