RBFF

General

X-Gal/Iptg Solution | X-Gal 溶液，开封即用 10 mL

Di: Amelia

To perform blue-white screening after transformation, X-Gal is added along with Isopropyl β-D-1-thiogalactopyranoside (IPTG), an inducer of lacZ ω gene expression. The blue colonies contain

Amresco N714-10ML X-GAL-IPTG READY SOLUTION X-GAL/IPTG 即用型溶液 | Amresco官网

Avantor is setting science in motion for a better world | Avantor Thermo Scientific X-Gal（5-溴-4-氯-3-吲哚基-β-D-半乳吡喃糖苷）是 β-半乳糖苷酶的惰性发色底物，该酶水解 X-Gal 产生无色的半乳糖和 4-氯-3-溴-靛蓝，形成深蓝色沉淀。IPTG 诱导 lacZ 基 Blue/White Screening of Bacterial Colonies X-Gal/IPTG PlatesPrepare 20 mg/ml X-Gal solution in DMF (See X-Gal Stock Solution Procedure). For reduced DMF toxicity in media,

X-Gal 溶液，开封即用 10 mL

Our X-Gal and IPTG products are intended to be used together for blue-white colony screening. 22μm membrane X-Gal (5-Bromo-4-Chloro-3-Indolyl-beta-D-Galactoside) is a chromogenic substrate for beta

Blue/White Screening of Bacterial Colonies X-Gal/IPTG PlatesPrepare 20 mg/ml X-Gal solution in DMF (See X-Gal Stock Solution Procedure). For reduced DMF toxicity in media,

Find iptg / x-gal solution and related products for scientific research at MilliporeSigma

βガラクトシダーゼX-Galはβ-ガラクトシダーゼの基質となり、加水分解すると青色を呈する。 したがって、 lacZ 欠損細胞を宿主としてpUC系プラスミドベクターDNAによる形質転換

  • IPTG Solution, ready-to-use 10 x 1.5 mL
  • What are X Gal and IPTG storage conditions?
  • Avantor is setting science in motion for a better world

Hier sollte eine Beschreibung angezeigt werden, diese Seite lässt dies jedoch nicht zu.

保存：-20℃ IPTG和X-Gal在涂板时无先后顺序要求。也可混匀后涂布，但IPTG与X-Gal的混合液不能加入到将要涂布的菌液中，否则容易使菌液致死。 solution is 因X-Gal & IPTG对各种载体的诱导活

X-Gal-Lösung, gebrauchsfertig 10 ml

Wrap the centrifuge tube in aluminum foil to protect from light. Note: The X-Gal solution is stable for 6-12 months unless improperly handled. We recommend

ジメチルホルムアミドに20 mg/mlになるように溶解する。 溶液は、遮光して－20℃で保存 1L of autoclaved （X-Galの入った培地は遮光して4℃で保存し、少なくとも1週間以内に使用すること）

qARTA Bio ProductsClick here for your FREE SAMPLE

  • Blue/White Screening of Bacterial Colonies X-Gal/IPTG Plates
  • X-Gal Solution, ready-to-use
  • X-GalとIPTGを添加できるスプレー
  • X-Gal-Lösung, gebrauchsfertig 10 ml

X-Gal/IPTG Solution – GenDEPOT

IPTG can be reconstituted in water. Make a stock of 100 mM in water and store working aliquots 가지는 vector로 형질전환된 균주를 선별할 at -20°C. X-gal can be reconstituted in DMSO, or in a 50:50 mix of DMSO and water. To do the

The IPTG solution has been formulated for consistent inactivation of the lac repressor. The IPTG solution can be used in conjunction with X-Gal for blue-white colony screening or by itself to IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli. The expression of cloned genes under the a) X-Gal included in agar: Add 5 mL of X-Gal stock solution and 5 ml of 0.1 M isopropyl-b-D-thiogalactoside (IPTG, BIO-37036) for each 1L of autoclaved media agar (e.g. LB agar)

Thermo Scientific X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose

Induction of the lacZ gene with IPTG leads to the hydrolysis of X-Gal and to the development of blue colonies (see Supporting data). X-Gal Solution, ready-to-use, is stable, 0.22 μm IBI’s IPTG/X-GAL Solution is a ready-to-use product packaged in an easy-to-use spray bottle Induction of the lacZ gene with IPTG leads to the hydrolysis of X-Gal and to the development of blue colonies. X-Gal Solution is a stable, 0.22μm membrane filtered solution formulated for

Stable solution of X-GAL and IPTG. Does not contain DMSO or DMF. Solution concentration is 10 mg/ml X-Gal and 8 mg/ml IPTG. Can X-gal be resuspended in dimethylsulfoxide (DMSO) instead of dimethylformamide (DMF)? How are X-gal plates prepared for blue/white screening? X-gal (also known as 5-bromo-4

Thermo Scientific X-Gal (5-Brom-4-Chlor-3-Indolyl-Beta-D-Galactopyranosid) ist ein inertes chromogenes Substrat für Beta-Galaktosidase, das X-Gal zu einer farblosen Galaktose und 4

Adding X-Gal and IPTG using the method mentioned above minimizes the risk of compound degradation compared to adding them directly to LB agar medium that may be too hot. X-Gal Solution, ready-to-use, is stable, 0.22 μm membrane filtered solution formulated for direct use in conjunction with IPTG for blue/white colony screening. 3. Spread 20 µL of X-Gal onto plates, then let adsorb again at 37°C. Q: What do I do if I forget to add X-Gal/IPTG to my media? If transformants are already on the plate and have grown visible

Thermo Scientific X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X Solution concentration is 10 mg/ml X-Gal and 8 mg/ml IPTG. Chromogenic substrate of β-Galactosidase used in combination with IPTG for detection of β-Galactosidase activity in

Thermo Scientific X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X X-Galを用いたブルー・ホワイトセレクションの原理とプロトコルを紹介します。ブルー・ホワイトセレクション（Blue/white selection; 青白選択）は、ブルー・ホワイトス

The X-gal solution should be protected from light. To make plates, add 50 ug/ml X-gal and 1 mM (0.24 mg/mL) IPTG to LB/agar that has been cooled down to

X-Gal staining is frequently used in the fields of molecular biology, genetics, and developmental biology to study gene expression and cell line tracing. [1] X-Gal Solution is provided as a

2% X-galの作り方です。 X-galは通称であり、 正式、名称は5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside (5-ブロモ-4-クロロ-3-インドリル-β-D-ガラクトピラノシド)です X-Gal은 β-galactosidase Gal and to the activity를 확인하기 위해 사용됩니다. lacZ 또는 lacZ α-peptide gene을 가지는 vector로 형질전환된 균주를 선별할 수 있습니다. 화학식 : C14H15BrCINO6 분자량 : 408.6